Bioinformatics II. Advanced Genome Informatics.

BCB/GDCB/STAT/COM S 594 [568] Brendel/Huang Spring 2007


Lecture times and locations

Tues, Thur 9:30-10:50, 1424 MBB

Tentative schedule (class notes can be viewed with Adobe Acrobat Reader)

DAY DATE CHAPTER TOPIC LECTURER
Tues Jan 9 0.1 Orientation
Vingron's Editorial, Bioinformatics 17, 389 [2001]
Berthou and Alcaraz, BMC Biolgoy: "...statistical practice is generally poor, even in the most renowned scientific journals ..." [2004]
Volker Brendel
Thur Jan 11 1.1 Sequence analysis with scores
(Karlin & Altschul (1990) PNAS 87: 2264
Karlin & Brendel (1992) Science 257: 39)
Volker Brendel
Tues Jan 16 1.2 Amino acid substitution scoring matrices
(Henikoff & Henikoff (1992) PNAS 89: 10915)
Volker Brendel
Thur Jan 18 1.3 Amino acid substitution scoring matrices: PAM model
Homework project #1
Volker Brendel
Tues Jan 23 2.1 Motifs: reguluar expressions, weight matrices, and profiles
Stormo (2000) Bioinformatics 16: 16
Henikoff & Henikoff (1994)J. Mol. Biol. 243: 574
Xiaoqiu Huang
Thur Jan 25 2.2 Profile alignments; PSI-BLAST
Altschul et al. (1997) Nucl. Acids Res. 17: 3389
Xiaoqiu Huang
Tues Jan 30 2.3 New directions in sequence alignment
Huang & Brutlag (2007) Nucl. Acids Res. 35: advance publication
Xiaoqiu Huang
Thur Feb 1 2.4 Identifcation of motifs I: EM-algorithm
Volker Brendel
Tues Feb 6 2.5 Identifcation of motifs II: Gibbs sampling and other approaches
Homework project #2
Volker Brendel
Thur Feb 8 3.1 Sequence models I (permutations; Markov models; pattern occurrence probabilities)
Volker Brendel
Tues Feb 13 3.2 Sequence models II (advanced Markov models)
Patchiness and correlations in DNA sequences
Volker Brendel
Thur Feb 15 4.1 Hidden Markov models - Theory I (Rabiner's Tutorial)
Volker Brendel
Tues Feb 20 4.2 Hidden Markov models - Theory II
Volker Brendel
Thur Feb 22 4.3 Applications I: Prokaryotic genome annotation
GENMARK: Parallel gene recognition for both DNA strands
GeneMark.hmm
GeneMarkS
Xiaoqiu Huang
Tues Feb 27 4.4 Applications I: Eukaryotic protein-coding gene identification
Prediction of complete gene structures in human genomic DNA
HW#2 due!
Xiaoqiu Huang
Thur Mar 1 4.5 Applications II: Other topics of eukaryotic genome annotation
Computational analysis of RNAs
Homework assignment #3
Xiaoqiu Huang
Tues Mar 6 5.1 Splice site prediction models
PMB36-573.pdf
NAR24-4709.pdf
NAR26-4748.pdf
Volker Brendel
Thur Mar 8 5.2 Gene identification by transcript mappting and comparative genomics: spliced alignment
Bioinformatics16-203.pdf
Bioinformatics20-1157.pdf
Volker Brendel
Tues Mar 13 SPRING BREAK
Thur Mar 15 SPRING BREAK
Tues Mar 20 6.1 Molecular Phylogeny I (basics)
HW#3 due!
Volker Brendel
Thur Mar 22 6.2 Molecular Phylogeny II (parsimony and distance matrix methods) Volker Brendel
Tues Mar 27 6.3 Molecular Phylogeny III (synonymous and nonsynonymouse substitutions I) Xiaoqiu Huang
Thur Mar 29 6.4 Molecular Phylogeny III (synonymous and nonsynonymouse substitutions II)
Goldman & Yang (1994) Mol. Biol. Evol. 11: 725
Xiaoqiu Huang
Tues Apr 3 7.1 Tools and resources for comparative plant genomics, with applications to the analysis of alternative pre-mRNA splicing
Wang & Brendel (2006) PNAS 103: 7175
Homework assignment #4
Volker Brendel
Thur Apr 5 7.2 Applications of Ka/Ks measures to genome analysis (exon finding, duplicated genes)
GenomeResearch12-198.pdf
Xiaoqiu Huang
Tues Apr 10 8.1 Recent methods for eukaryotic genome annotation
Sparks & Brendel (2005) Bioinformatics 21 Suppl. 3: iii20
Bioinformatics17-S140.pdf
Volker Brendel
Thur Apr 12 8.2 Working groups: Designing a comprehensive genome annotation work flow I
Volker Brendel
Tues Apr 17 8.3 Working groups: Designing a comprehensive genome annotation work flow II
GenomeBiology7-S9.pdf
BioDAS
Volker Brendel
Thur Apr 19 8.4 Databases for Practical Bioinformatics: MySQL, xGDB, UCSC Genome Browser, and Ensembl
Matthew Wilkerson
Tues Apr 24 Review I: Genome Annotation - Practice at PlantGDB
Matthew Wilkerson and Jon Duvick
Thur Apr 26 Review II: Genome Informatics
Xiaoqiu Huang and Matthew Wilkerson
Tues May 1 Orals I
Volker Brendel and Xiaoqiu Huang
Thur May 3 Orals II
Volker Brendel and Xiaoqiu Huang